Pregnenolone ELISA Kit

Supplier: Elabscience
Product Type: ELISA Kit
Size: 96T
Description: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Pregnenolone. During the reaction, Pregnenolone in the sample or standard competes with a fixed amount of  Pregnenolone on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  Pregnenolone. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of   Pregnenolone in the samples is then determined by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Competitive
Target Name: Pregnenolone
Target Synonmy:
UNIProt ID:
Target Species: Universal
Detection Range: 0.781--50ng/mL
Sensitivity: 0.469ng/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant

Product Link: http://www.elabscience.com/p-pregnenolone elisa kit-17628.html

Manaul Link: http://www.elabscience.com/viewpdf-17628-Elabscience-E-EL-0086.pdf