Recombinant Mouse Legumain/Asparaginyl Endopeptidase Protein

Supplier: Elabscience Product Name: Recombinant Mouse Legumain/Asparaginyl Endopeptidase Protein Catalog No. PKSM041191 Product Type: recombinant protein Size: 50 ug Activity: Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin(Z-AAN-AMC).The specific activity is > 350 pmoles/min/?g.(Activation description: The enzyme achieves its activity under acidic pH) Protein Construction: Recombinant Mouse Legumain/Asparaginyl Endopeptidase is produced by our Mammalian expression system and the target gene encoding Val18-Tyr435 is expressed with a 6His tag at the C-terminus. Sequence: Val 18-Tyr 435 Fusion Tag: C-6His Accession: NP_035305.1 Species: Mouse Expressed Host: Human Cells Shipping Conditions: Liquid. It is shipped out with blue ice. Purity: ?95% as determined by reducing SDS-PAGE. Endotoxin: <1.0 EU per ?g as determined by LAL test. Stability: Store at < -20?, stable for 6 monthshs after receipt.Please minimize freeze-thaw cycles Molecular Weight: 48.7 kDa Applied MM: 60kDa Formulation: Supplied as a 0.2 ?m filtered solution of 20mM Tris,150mM NaCl,20% Glycerol,pH8.0. Reconstitution: Please refer to it for detailed information. Background: Mouse Legumain,also known as LGMN, is a cysteine protease belonging to peptidase family C13 and is expressed in kidney and placenta abundantly. LGMN has a strict specificity for hydrolysis of asparaginyl bonds. It can also cleave aspartyl bonds slowly, especially under acidic conditions. The mammalian legumain is involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. It plays a role in the regulation of cell proliferation via its role in EGFR degradation. Legumain deficiency causes the accumulation of pro-Cathepsins B, H and L, another group of lysosomal cysteine proteases. Overexpression of Legumain in tumors is significant for invasion/metastasis. Mammalian legumain is inhibited by iodoacetamide and maleimides. Legumain activation appears to be autocatalytic and can be triggered by acidic pH.