Recombinant Mouse Carboxypeptidase A2 Protein

Supplier: Elabscience Product Name: Recombinant Mouse Carboxypeptidase A2 Protein Catalog No. PKSM041201 Product Type: recombinant protein Size: 50 ug Activity: Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of DTNB.The specific activity is >4000 pmoles/min/?g. Protein Construction: Recombinant Mouse Carboxypeptidase A2 is produced by our Mammalian expression system and the target gene encoding Gln17-Tyr417 is expressed with a 6His tag at the C-terminus. Sequence: Gln17-Tyr417 Fusion Tag: C-6His Accession: Q504N0 Species: Mouse Expressed Host: Human Cells Shipping Conditions: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Purity: ?95% as determined by reducing SDS-PAGE. Endotoxin: <1.0 EU per ?g as determined by LAL test. Stability: Lyophilized protein should be stored at < -20?, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20? for 3 months Molecular Weight: 46.2 kDa Applied MM: 41-50 kDa Formulation: Lyophilized from a 0.2 ?m filtered solution of 20mM TrisHCl, 150mM NaCl, pH8.0. Reconstitution: Please refer to it for detailed information. Background: Mouse carboxypeptidase A2(CPA2) is a secreted pancreatic procarboxy -peptidase which belongs to the peptidase M14 family. CPA2 consists of a signal peptide, a pro region and a mature chain. It can be activated after cleavage of the pro peptide. CPA2 cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan.