Supplier: Cloud-Clone Corp.
Product Type: ELISA Kit
Size: 96T
Item Name: Tumor Necrosis Factor Alpha
Alternative Names: DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
Description: The microplate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha (TNFa). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Alpha (TNFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tumor Necrosis Factor Alpha (TNFa) level in the sample or standard.;
Research Area: Cytokine;Tumor immunity;Infection immunity;
Assay Type: Double-antibody Sandwich
Applications: Chemiluminescent immunoassay for Antigen Detection.
Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Organism Species: Rattus norvegicus (Rat)
Detection Sensitivity: < 0.51pg/mL
Detection Range: 1.37-1,000pg/mL
Assay Length: 2 hours
Product Link: http://www.cloud-clone.com/products/SCA133Ra.html