Supplier: Cloud-Clone Corp.
Product Type: ELISA Kit
Size: 96T
Item Name: Dopamine
Alternative Names: 2-(3,4-Dihydroxyphenyl)ethylamine; 3,4-Dihydroxyphenethylamine; 3-Hydroxytyramine; Intropin; Revivan; Oxytyramine; Dopamine Hydrochloride
Description: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Dopamine (DA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Dopamine (DA) and unlabeled Dopamine (DA) (Standards or samples) with the pre-coated antibody specific to Dopamine (DA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Dopamine (DA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Dopamine (DA) in the sample.
Research Area: Signal transduction;Cardiovascular biology;Reproductive science;Neuro science;
Assay Type: Competitive Inhibition
Applications: Enzyme-linked immunosorbent assay for Antigen Detection.
Sample Types: serum, plasma and other biological fluids
Organism Species: Pan-species (General)
Detection Sensitivity: < 4.71pg/mL
Detection Range: 12.35-1000pg/mL
Assay Length: 2 hours
Product Link: http://www.cloud-clone.com/products/CEA851Ge.html