Rat Angiotensin I Converting Enzyme (ACE) ELISA Kit

Supplier: Cloud-Clone Corp.

Product Type: ELISA Kit

Size: 96T

Item Name: Angiotensin I Converting Enzyme

Alternative Names: CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme (ACE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme (ACE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin I Converting Enzyme (ACE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiotensin I Converting Enzyme (ACE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Enzyme & Kinase;Cardiovascular biology

Assay Type: Double-antibody Sandwich

Applications: Enzyme-linked immunosorbent assay for Antigen Detection.

Sample Types: Serum, plasma, cerebrospinal fluid and other biological fluids

Organism Species: Rattus norvegicus (Rat)

Detection Sensitivity: < 0.122ng/mL

Detection Range: 0.312-20ng/mL

Assay Length: 2 hours

Product Link: http://www.cloud-clone.com/products/SEA004Ra.html