Recombinant Mouse Triggering Receptor Expressed on Myeloid Cells 2b/TREM-2b Protein(C-Fc)

Supplier: Elabscience Product Name: Recombinant Mouse Triggering Receptor Expressed on Myeloid Cells 2b/TREM-2b Protein(C-Fc) Catalog No. PKSM041175 Product Type: recombinant protein Size: 50 ug Activity: Protein Construction: Recombinant Mouse Triggering Receptor Expressed on Myeloid Cells 2b is produced by our Mammalian expression system and the target gene encoding Leu19-Pro168 is expressed with a Fc tag at the C-terminus. Sequence: Leu19-Pro168 Fusion Tag: C-Fc Accession: Q99NH8 Species: Mouse Expressed Host: Human Cells Shipping Conditions: The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below. Purity: ?95% as determined by reducing SDS-PAGE. Endotoxin: <1.0 EU per ?g as determined by LAL test. Stability: Lyophilized protein should be stored at < -20?, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20? for 3 months Molecular Weight: 43.7 kDa Applied MM: 60kDa Formulation: Lyophilized from a 0.2 ?m filtered solution of PBS, pH7.4. Reconstitution: Please refer to the printed manual for detailed information. Background: Triggering receptor expressed on myeloid cells-2 (TREM-2) is a cell surface receptor primarily expressed on macrophages, osteoclasts, microglia and dendritic cells. TREM-2 is one member of the TREM family, inhibiting the releasing of inflammatory mediators, so it is an important in vivo anti-inflammatory receptor. TREM-2 consists of an 18 aa signal sequence, a 153 aa extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. A soluble form of TREM-2 (TREM-2b) created by alternate splicing diverges at aa 161. TREM-2 transduces intracellular signals through the adaptor DAP12. After binding of TREM-2 with ligand, the TREM-2/DAP12 (dead-cell-activated-receptor-associated protein)-mediated signal transduction pathway causes a series of intracellular protein tyrosine phosphorylation reactions and enzymatic reactions, which then activate the myeloid cells and participate T cell responses.