Recombinant Mouse Tumor Necrosis Factor/TNF?Protein

Supplier: Elabscience Product Name: Recombinant Mouse Tumor Necrosis Factor/TNF?Protein Catalog No. PKSM041176 Product Type: recombinant protein Size: 50 ug Activity: Measured in a cytotoxicity assay using L?929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is less than 0.08 ng/ml. Protein Construction: Recombinant Mouse Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Asp89-Leu235 is expressed. Sequence: Asp89-Leu235 Fusion Tag: Accession: P06804 Species: Mouse Expressed Host: E.coli Shipping Conditions: The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below. Purity: ?95% as determined by reducing SDS-PAGE. Endotoxin: <1.0 EU per ?g as determined by LAL test. Stability: Lyophilized protein should be stored at < -20?, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20? for 3 months Molecular Weight: 16.4 kDa Applied MM: 14 kDa Formulation: Lyophilized from a 0.2 ?m filtered solution of PBS, pH 7.4. Reconstitution: Please refer to the printed manual for detailed information. Background: Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.