Recombinant Mouse ?-Nerve Growth Factor/?-NGF (Ser122-Gly241) Protein

Supplier: Elabscience Product Name: Recombinant Mouse ?-Nerve Growth Factor/?-NGF (Ser122-Gly241) Protein Catalog No. PKSM041189 Product Type: recombinant protein Size: 50 ug Activity: Measured in a cell proliferation assay using TF?1 human erythroleukemic cellsThe ED50 for this effect is less than 2 ng/ml. Protein Construction: Recombinant Mouse beta-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Ser122-Gly241 is expressed. Sequence: Ser122-Gly241 Fusion Tag: Accession: P01139 Species: Mouse Expressed Host: E.coli Shipping Conditions: The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below. Purity: ?95% as determined by reducing SDS-PAGE. Endotoxin: <1.0 EU per ?g as determined by LAL test. Stability: Lyophilized protein should be stored at < -20?, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20? for 3 months Molecular Weight: 13.5 kDa Applied MM: 14 kDa Formulation: Lyophilized from a 0.2 ?m filtered solution of 20mM Tris,150mM NaCl,pH8.0. Reconstitution: Please refer to the printed manual for detailed information. Background: NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. ?-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor ?-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems.