Recombinant Mouse T cell Immunoreceptor with Ig and ITIM domains Protein

Supplier: Elabscience Product Name: Recombinant Mouse T cell Immunoreceptor with Ig and ITIM domains Protein Catalog No. PKSM041197 Product Type: recombinant protein Size: 50 ug Activity: Immobilized mouse TIGIT-His at 10 ?g/ml (100 ?l/well) can bind mouse PVR-Fch, The EC50 of mouse PVR-Fch is 0.31-0.73 ?g/ml. Protein Construction: Recombinant Mouse T cell Immunoreceptor with Ig and ITIM domains is produced by our Mammalian expression system and the target gene encoding Gly26-Phe135 is expressed with a 6His tag at the C-terminus. Sequence: Gly26-Phe135 Fusion Tag: C-6His Accession: NP_001139797 Species: Mouse Expressed Host: Human Cells Shipping Conditions: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Purity: ?95% as determined by reducing SDS-PAGE. Endotoxin: <1.0 EU per ?g as determined by LAL test. Stability: Lyophilized protein should be stored at < -20?, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20? for 3 months Molecular Weight: 13.3 kDa Applied MM: 18-22 kDa Formulation: Lyophilized from a 0.2 ?m filtered solution of PBS, pH7.4. Reconstitution: Please refer to it for detailed information. Background: T cell immunoreceptor with Ig and ITIM domains (TIGIT), also called WUCAM, VSIG9 and Vstm3, is a member of the CD28 family within the Ig superfamily of proteins. TIGIT contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM), and is expressed on regulatory, memory, activated T cells and NK cells. TIGIT binds to CD155(PVR) that appear on dendritic cells (DC), macrophages and endothelium with high affinity, and CD112(PVRL2) with lower affinity, but not CD113 (PVRL3). TIGIT-Fc fusion protein could interact with PVR on DC and enhance the secretion of IL-10, but inhibit the macrophage activation. Mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges, while knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses.