Recombinant Mouse Cytosolic non-specific dipeptidase Protein

Supplier: Elabscience Product Name: Recombinant Mouse Cytosolic non-specific dipeptidase Protein Catalog No. PKSM041231 Product Type: recombinant protein Size: 50 ug Activity: Measured by its ability to cleave carnosine (?-Ala-His) in a two step assay (mouse CNDP2 concentration 10 ?g/ml). The specific activity is >40 pmoles/min/?g. Protein Construction: Recombinant Mouse Cytosolic non-specific dipeptidase is produced by our Mammalian expression system and the target gene encoding Met1-Asn475 is expressed with a 6His tag at the C-terminus. Sequence: Met 1-Asn 475 Fusion Tag: C-6His Accession: NP_075638.2 Species: Mouse Expressed Host: Human Cells Shipping Conditions: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Purity: ?95% as determined by reducing SDS-PAGE. Endotoxin: <1.0 EU per ?g as determined by LAL test. Stability: Store at < -20?, stable for 6 monthshs after receipt.Please minimize freeze-thaw cycles Molecular Weight: 53.8 kDa Applied MM: 56-60 kDa Formulation: Supplied as a 0.2 ?m filtered solution of 20mM TrisHCl, 150mM NaCl,10% Glycerol, pH8.0. Reconstitution: Please refer to it for detailed information. Background: Mouse cytosolic non-specific dipeptidase(CNDP2) is a cytoplasm protein which belongs to the peptidase M20A family. CNDP2 has 2 Isoform: Isoform 1 is ubiquitously expressed with higher levels in kidney and liver (at protein level). Isoform 2 is expressed in fetal tissues, it is only expressed in adult liver and placental tissues. CNDP2 hydrolyzes a variety of dipeptides including L-carnosine and has a strong preference for Cys-Gly. It is a cytosolic enzyme that can hydrolyze carnosine to yield l-histidine and beta-alanine. CNDP2 is highly expressed in the histaminergic neurons in the tuberomammillary nucleus, implying that it may supply histidine to histaminergic neurons for histamine synthesis. It may play a role as tumor suppressor in hepatocellular carcinoma (HCC) cells.