Recombinant Mouse DNAX Accessory Molecule-1 Protein

Supplier: Elabscience Product Name: Recombinant Mouse DNAX Accessory Molecule-1 Protein Catalog No. PKSM041233 Product Type: recombinant protein Size: 50 ug Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse DNAM1 at 2 ?g/ml (100 ?l/well) can bind mouse CD155 with a linear ranger of 6.4-160 ng/ml. Protein Construction: Recombinant Mouse DNAX Accessory Molecule-1 is produced by our Mammalian expression system and the target gene encoding Glu19-Pro254 is expressed with a 6His tag at the C-terminus. Sequence: Met 1-Pro 254 Fusion Tag: C-6His Accession: NP_848802.2 Species: Mouse Expressed Host: Human Cells Shipping Conditions: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Purity: ?95% as determined by reducing SDS-PAGE. Endotoxin: <1.0 EU per ?g as determined by LAL test. Stability: Lyophilized protein should be stored at < -20?, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20? for 3 months Molecular Weight: 27.6 kDa Applied MM: 35-50 kDa Formulation: Lyophilized from a 0.2 ?m filtered solution of PBS, pH7.4. Reconstitution: Please refer to it for detailed information. Background: Mouse DNAX accessory molecule-1(DNAM-1) is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. As an activating receptor, it interacts with the ligands CD155 and CD112, and activates natural killer (NK) cells via its immu-noreceptor tyrosine-based activatory motif (ITAM). Mature mouse DNAM-1 has extracellular domain (ECD) that contains two Ig-like C2-set domains, and possesses a cytoplasmic region that contains motifs for binding PDZ domains. DNAM-1 is expressed on several lymphoid and myeloid cell types and interacts with CD155/PVR and Nectin-2/CD112. Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells, induces dendritic cell maturation, initiates megakaryocyte and activated platelet adhesion to vascular endothelial cells, and stimulates monocyte extravasation? Conversely, it inhibits the formation of osteoclasts. Platelet-endothelium interactions that are mediated by DNAM-1 enable the metastasis of tumor cells to the lung.