Recombinant Mouse Tumor Necrosis Factor Receptor II Protein

Supplier: Elabscience Product Name: Recombinant Mouse Tumor Necrosis Factor Receptor II Protein Catalog No. PKSM041237 Product Type: recombinant protein Size: 50 ug Activity: Measured by its ability to inhibit TNF?-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 1-3 ?g/mL in the presence of 0.1 ng/mL of recombinant mouse TNF?. Protein Construction: Recombinant Mouse Tumor Necrosis Factor Receptor II is produced by our Mammalian expression system and the target gene encoding Val23-Gly258 is expressed with a 6His tag at the C-terminus. Sequence: Met 1-Gly 258 Fusion Tag: C-6His Accession: NP_035740.2 Species: Mouse Expressed Host: Human Cells Shipping Conditions: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Purity: ?95% as determined by reducing SDS-PAGE. Endotoxin: <1.0 EU per ?g as determined by LAL test. Stability: Lyophilized protein should be stored at < -20?, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20? for 3 months Molecular Weight: 26.4 kDa Applied MM: 42 kDa Formulation: Lyophilized from a 0.2 ?m filtered solution of PBS, pH7.4. Reconstitution: Please refer to it for detailed information. Background: Tumor Necrosis Factor Receptor Superfamily Member 1B (TNFRSF1B) is a member of the Tumor Necrosis Factor Receptor Superfamily. TNFRSF1B contains four TNFR-Cys repeats. TNFRSF1B can be cleaved into the following 2 chains: Tumor necrosis factor receptor superfamily member 1b and membrane form and Tumor necrosis factor-binding protein 2. TNFRSF1B is a receptor with high affinity for TNFSF2/TNF-? and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-?. TNFRSF1B mediates most of the metabolic effects of TNF-?. TNF-?-induced apoptosis suggests that it regulates TNF-? function by antagonizing its biological activity.