Recombinant Mouse Cathepsin D Protein

Supplier: Elabscience Product Name: Recombinant Mouse Cathepsin D Protein Catalog No. PKSM041238 Product Type: recombinant protein Size: 50 ug Activity: Protein Construction: Recombinant Mouse Cathepsin D is produced by our Mammalian expression system and the target gene encoding Ile21-Leu410 is expressed with a 6His tag at the C-terminus. Sequence: Met 1-Leu 410 Fusion Tag: C-6His Accession: NP_034113.1 Species: Mouse Expressed Host: Human Cells Shipping Conditions: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Purity: ?95% as determined by reducing SDS-PAGE. Endotoxin: <1.0 EU per ?g as determined by LAL test. Stability: Lyophilized protein should be stored at < -20?, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20? for 3 months Molecular Weight: 43.9 kDa Applied MM: 56kDa Formulation: Lyophilized from a 0.2 ?m filtered solution of 20mM MES,150mM NaCl,pH5.5. Reconstitution: Please refer to it for detailed information. Background: CTSD localizes to the lysosome and consists of a light chain and a heavy chain. CTSD is expressed in epithelial cells as well as in macrophages.CTSD is a lysosomal aspartyl protease that depends critically on protonation of its active site Asp residue and gets activated at pH 5 in endosome of hepatocytes. It has been suggested to facilitate cancer cell migration and invasion by digesting the basement membrane, extracellular matrix and xonnective tissue. In addition, CTSD has been used as a breast cancer tumor marker.