Mouse Monoclonal Antibody to MCL-1

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000
Size: 100 ul, 1mg/ml
Species Reactivity: Human
Clone: 8C6;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of human MCL-1 expressed in E. Coli. ;
Formulation: Purified antibody in PBS containing 0.03% sodium azide.
MW: 37kDa
Storage: 4C; -20C for long term storage
Supplier link: http://www.promab.com/index.php?main_page=product_info&products_id=58
Reference: 1. Ota, N. et al. J. Hum. Genet. 2000. 46: 254-269. ; 2. Schwertfeger KL, Ryder JW, Anderson SM J Mammary Gland Biol Neoplasia 2000, 3 : 236-251. ; ; ; ; ; ;

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