Mouse Monoclonal Antibody to RAG2

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000
Size: 100 ul, 1mg/ml
Species Reactivity: Human
Clone: 4D5;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of human RAG2(350-527aa) expressed in E. Coli.
Formulation: Ascitic fluid containing 0.03% sodium azide. ;
MW: 59kDa
Storage: 4C; -20C for long term storage
Supplier link: http://www.promab.com/index.php?main_page=product_info&products_id=431
Reference: 1. J Biol Chem. 2004 Sep 10;279(37):38360-8. ; 2. Immunity. 2005 Aug;23(2):203-12. ; 3. J Clin Invest. 2010 Apr 1;120(4):1337-44. doi: 10.1172/JCI41305.

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