Brain-derived neurotrophic factor, pro- (proBDNF), Human, Mouse, Rat, Rapid™ ELISA assay-1 Plate Kit

Supplier: Biosensis Product Type: ELISA Assay Description: The Biosensis proBDNF Rapid^TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, human serum and EDTA-plasma only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples.

This ELISA kit consists of a pre-coated monoclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance.

This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced in mammalian cells by Biosensis and validated against externally available proBDNF proteins.

Note that accurate proBDNF quantification in human serum and EDTA-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately.

This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures. Unit Size: 1 Plate Kit Antiboy Host Species: Antibody Isotype: Applications: ELISABioActivity: Clone Name: Conjugate: Detection Method: Colorimetric Species Reactivity: Human,Mouse,Rat Expiration Date: 12 months from purchase. Storage Temperature: 2-8°C Shipping Tempereature: 2-8°C (on cold packs) Product URL: www.biosensis.com/probdnf-rapid-elisa-assay.html